Additional Information:
UFHPL Epic order code: LAB90
CPT Code(s):
Collection Procedure:
Specimen Requirements:
Type: Peripheral blood
Container/Tube: EDTA whole blood
Sample Volume: 0.5mL
- Ambient: 8 hours
- Refrigerated: 7 days
- Frozen: 3 months
Methodology:
In a pretreatment step, whole blood is mixed with the Hemolyzing reagent in a 1:100 dilution and the resultant hemolyate is used. The concentration of both HbA1c and total hemoglobin are determined. The HbA1c/Total Hemoglobin ratio is expressed as %HbA1c. Total hemoglobin reagent is used to measure total hemoglobin concentration by a colorimetric method. Change in absorbance is measure at 570/660 nm. HbA1c reagent is used to measure hemoglobin A1c concentration by a turbidimetric immunoinhibition method. In the reaction, hemoglobin A1c antibodies combine with HbA1c from the sample to form soluble antigen-antibody complexes. Polyhaptens from the reagent then bind with the excess antibodies and the resulting agglutinated complex is measured turbidimetrically. Change in absorbance is measured at 340/700 nm.
Reported:
Within 24 hours